Molecular Techniques in Physiology — MCQs

Techniques used for protein expression proteomics study include:

Which of the following is used to detect abnormal gene sequences EXCEPT?

Which one of the following enzymes is obtained from Thermus aquaticus bacterium that is heat stable and used in PCR at high temperature?

All are examples of negative feedback except

A novel drug is designed to treat a genetic disorder caused by a nonsense mutation in the dystrophin gene. The drug works by allowing the ribosome to skip over the premature stop codon and continue translation. Evaluation of this therapeutic strategy reveals partial restoration of dystrophin protein with 60% of normal length but sufficient function. What is the most critical molecular consideration in determining if this approach will be clinically beneficial?

A 45-year-old woman with breast cancer is being treated with trastuzumab (Herceptin). Laboratory analysis of her tumor cells shows 100-fold amplification of a specific gene. Analysis of the signal transduction pathway reveals constitutive activation of PI3K/AKT pathway. Which receptor is most likely overexpressed?

A 6-month-old infant presents with severe anemia, jaundice, and hepatosplenomegaly. Hemoglobin electrophoresis shows predominantly HbF (fetal hemoglobin) with absent HbA. Which molecular defect best explains this clinical picture?

Which of the following is not a channelopathy?

What is the composition of epithelial sodium channels?