Parasitology Practice Questions

Q: Sabin Feldman dye test is used for diagnosis of which of the following condition?

Toxoplasmosis. ***Toxoplasmosis*** The **Sabin-Feldman dye test** is a **serological assay** used to detect specific IgG antibodies against *Toxoplasma gondii*, the causative agent of toxoplasmosis. It measures the ability of antibodies in a patient's serum to prevent the cytoplasmic staining of live toxoplasma tachyzoites by methylene blue dye, indicating an **active immune response** to the parasite. This classic test has high sensitivity and specificity for detecting toxoplasma antibodies and is considered the gold standard serological test, though it has been largely replaced by ELISA and IFA in routine practice due to the requirement for live organisms. *Botulism* Botulism is diagnosed through **toxin detection** in serum, stool, or food samples using mouse bioassay, or by culturing *Clostridium botulinum* from clinical specimens. The Sabin-Feldman dye test is not relevant for the diagnosis of botulism, which is a **neuroparalytic disease** caused by botulinum neurotoxin blocking acetylcholine release at neuromuscular junctions. *Sarcoidosis* Sarcoidosis is a multisystem **granulomatous disease** diagnosed primarily by **tissue biopsy** showing non-caseating granulomas along with compatible clinical and radiological findings. Supportive tests include elevated serum ACE levels and Kveim test (historical). There is no serological test like the Sabin-Feldman dye test associated with the diagnosis of sarcoidosis, as it is not an infectious disease requiring antibody detection. *Yellow fever* Yellow fever is a **viral hemorrhagic disease** caused by a flavivirus, diagnosed by detecting viral RNA through RT-PCR or specific IgM antibodies in the acute phase of infection using ELISA or immunofluorescence. The Sabin-Feldman dye test is not used for viral infections like yellow fever, as it specifically targets **toxoplasma antibodies** and has no role in arboviral disease diagnosis.

Q: Identify the organism related to the blood smear image.

Plasmodium falciparum. ***Plasmodium falciparum*** - This organism is characterized by **delicate, ring-form trophozoites** and occasionally **banana-shaped gametocytes** in red blood cells. - The presence of multiple rings per red blood cell and **applique forms** (rings on the periphery of the red blood cell) is highly suggestive of *P. falciparum* infection. - Blood smears showing **high parasitemia** with predominantly ring forms are pathognomonic for *P. falciparum* malaria. *Salmonella Typhi* - This is a **gram-negative bacterium** that causes typhoid fever, primarily affecting the gastrointestinal tract. - It does not present as **intraerythrocytic parasites** on a blood smear. - Diagnosis is made through **blood culture** or bone marrow culture, not blood smear microscopy. *Toxoplasma gondii* - This is an **obligate intracellular parasite** that typically forms **tissue cysts** (e.g., brain, muscle) and is not found as intraerythrocytic forms. - Tachyzoites may be seen in blood in acute infection, but appear as **free extracellular** or intracellular forms in leukocytes, not within red blood cells. - Diagnosis is typically via **serology** or PCR, not routine blood smear examination. *Treponema pallidum* - This is a **spirochete bacterium** responsible for syphilis. - It is too thin to be seen on routine blood smears and is typically identified using **dark-field microscopy** or **serological tests** (VDRL, RPR, TPPA). - Does not appear as intraerythrocytic forms on blood smear.

Q: Which stage of Leishmania is found in spleen aspirate of a patient?

Amastigote stage. ***Amastigote stage*** - The **amastigote stage** is the **intracellular, non-flagellated form** of Leishmania that replicates within **macrophages** of the vertebrate host, including humans. - In visceral leishmaniasis, these **amastigotes** are abundant in organs like the **spleen**, liver, and bone marrow, making them detectable in spleen aspirates. *Promastigote stage* - The **promastigote stage** is the **extracellular, flagellated form** of Leishmania found in the digestive tract of the **sand fly vector**. - It is typically **not found** in human tissues or spleen aspirates. *Epimastigote stage* - The **epimastigote stage** is a flagellated form of **trypanosomes** (e.g., *Trypanosoma cruzi*) but **not Leishmania**. - It is found in the **vector's gut** for trypanosomes and is not part of the Leishmania life cycle in humans. *Trypomastigote stage* - The **trypomastigote stage** is another flagellated form characteristic of **trypanosomes** (e.g., *Trypanosoma cruzi* and *Trypanosoma brucei*). - It circulates in the **blood of the vertebrate host** for trypanosomes and is not observed in Leishmania infections within human organs like the spleen.

Q: Calabar swelling is produced by?

Loa loa. ***Loa loa*** - **Calabar swellings** are a pathognomonic feature of **loiasis**, caused by the **filarial nematode** *Loa loa*. - These are **transient, non-tender, subcutaneous edemas** that can reappear in different locations due to the migration of the adult worm. *Onchocerca volvulus* - This parasite causes **onchocerciasis** (river blindness), which is characterized by **subcutaneous nodules** (onchocercomas) and **dermatitis**, not migratory swellings. - Ocular involvement leading to blindness is a significant complication. *Brugia malayi* - This is one of the causes of **lymphatic filariasis**, primarily leading to **lymphedema** and **elephantiasis** in the lower limbs. - It does not typically cause Calabar swellings. *Wuchereria bancrofti* - This is the most common cause of **lymphatic filariasis**, also resulting in **lymphedema** and **elephantiasis**, particularly in the legs and genitalia. - It is not associated with migratory Calabar swellings.

Q: Pulmonary eosinophilia is found in infection with ?

Strongyloides. ***Strongyloides*** - **Strongyloides stercoralis** can cause **pulmonary eosinophilia** (Löffler's syndrome) as larvae migrate through the lungs [1]. - This migration is part of the parasite's life cycle, leading to an inflammatory response with eosinophil infiltration [1]. *Babesia* - **Babesia** species primarily infect **red blood cells** and cause **hemolytic anemia**, resembling malaria. - They are transmitted by ticks and do not typically cause pulmonary eosinophilia. *Malaria* - **Malaria**, caused by Plasmodium parasites, is known for infecting **red blood cells** and causing cyclical fevers, chills, and anemia. - It does not typically present with pulmonary eosinophilia as a characteristic feature. *Trypanosoma* - **Trypanosoma** species cause **sleeping sickness** (African trypanosomiasis) and **Chagas disease** (American trypanosomiasis). - These infections affect the bloodstream, lymphatic system, and various organs, but pulmonary eosinophilia is not a hallmark.

Q: Which of the following is the causative organism of Acanthamoeba keratitis?

Acanthamoeba. ***Acanthamoeba*** - **Acanthamoeba keratitis** is a serious and painful infection of the eye's cornea caused by species of the **free-living amoeba Acanthamoeba**. - This infection is commonly associated with contact lens wear, particularly when proper hygiene and disinfection practices are not followed. *Plasmodium* - **Plasmodium** species are the causative agents of **malaria**, a mosquito-borne infectious disease affecting red blood cells and the liver. - It does not cause eye infections like keratitis and is transmitted through the bite of an infected Anopheles mosquito. *Toxoplasma* - **Toxoplasma gondii** is an intracellular parasitic protozoon that causes **toxoplasmosis**, which can manifest as chorioretinitis, encephalitis, or lymphadenopathy. - While it can affect the eye (**ocular toxoplasmosis**), it causes retinitis or chorioretinitis, not keratitis. *W. bancrofti* - **Wuchereria bancrofti** is a filarial nematode that causes **lymphatic filariasis**, an infection leading to elephantiasis and other lymphatic disorders. - It primarily affects the lymphatic system and subcutaneous tissues, not the cornea.

Q: Promastigote form of Leishmania is found in which part of sandfly?

GIT. **GIT** - The **promastigote form** of *Leishmania* is found in the **gastrointestinal tract (GIT)** of the sandfly, specifically in the midgut. - After ingesting infected blood, the amastigotes transform into promastigotes and multiply in the sandfly's gut before migrating to the proboscis. *Lymph node* - **Lymph nodes** are part of the human host's immune system and are not where *Leishmania* promastigotes develop in the sandfly. - In humans, *Leishmania* primarily infects **macrophages** and transforms into the **amastigote form**. *Spleen* - The **spleen** is a major organ of the human immune system, where *Leishmania* **amastigotes** can multiply after infecting macrophages, particularly in visceral leishmaniasis. - It is not a site for promastigote development within the sandfly vector. *Bone marrow* - The **bone marrow** is another site within the human host where *Leishmania* **amastigotes** can be found, especially in cases of visceral leishmaniasis. - The sandfly's life cycle for *Leishmania* promastigotes does not involve the bone marrow.

Q: A 52-year-old male with HIV presents with profuse, watery diarrhea of 5 days’ duration. A biopsy of the small intestine is shown here. What is the most likely cause of this patient’s symptoms?

Cryptosporidium spp.. ***Cryptosporidium spp.*** - The image shows numerous **small, round, purple-stained organisms** adhering to the brush border of intestinal epithelial cells, which are characteristic of *Cryptosporidium* oocysts. - In an **HIV-positive patient** with profuse, watery diarrhea, *Cryptosporidium* is a common opportunistic infection that can cause severe and prolonged symptoms, especially when CD4 count is <200 cells/μL. - Diagnosis is confirmed by identifying **acid-fast oocysts** (3-6 μm) attached to the intestinal epithelial surface. *Giardia lamblia* - *Giardia* typically presents as pear-shaped **trophozoites** with flagella or oval **cysts** in the intestinal lumen, which are much larger (10-20 μm) than the organisms seen in this biopsy. - While *Giardia* can cause diarrhea in immunocompromised individuals, the morphology and location (attached to brush border vs. free in lumen) do not match. *Cystoisospora belli* - *Cystoisospora* (formerly *Isospora belli*) is another important cause of chronic diarrhea in HIV patients but appears as **large, elongated oocysts** (20-30 μm) that are acid-fast positive. - The organisms are typically found **within enterocytes** or in the lumen, not as small spherical structures on the brush border surface as seen here. *Cyclospora cayetanensis* - *Cyclospora* causes watery diarrhea in immunocompromised patients and appears as **round oocysts** (8-10 μm), larger than *Cryptosporidium*. - The oocysts are typically found in the **intestinal lumen** and stain variably with acid-fast staining, but the small size and surface attachment pattern in this image are more consistent with *Cryptosporidium*.

Q: What is a distinguishing feature of E. histolytica compared to E. dispar?

Ingestion of red blood cells by trophozoites. ***Ingestion of red blood cells by trophozoites*** - **E. histolytica** is a pathogenic amoeba that invades intestinal mucosa and is **hematophagous** (ingests RBCs) - The presence of **ingested erythrocytes within trophozoites** is a key distinguishing feature that suggests *E. histolytica* rather than *E. dispar* - *E. dispar* is **non-pathogenic** and does not typically ingest RBCs, as it does not invade tissues - While not 100% specific (some E. histolytica may not show RBCs, and molecular methods are gold standard), **finding RBCs in trophozoites is clinically significant** for differentiating these morphologically identical species *Presence of cysts with 1-4 nuclei* - Both *E. histolytica* and *E. dispar* form cysts with **1-4 nuclei** with identical morphology - The cyst stages of these two species are **morphologically indistinguishable** under light microscopy - This feature **cannot be used** to differentiate between the two species *Presence of pseudopodia* - Both species are amoebae and possess **pseudopodia** for motility during the trophozoite stage - This is a **general characteristic of all amoebae** and does not distinguish these species *Presence of blunt pseudopodia* - Both *E. histolytica* and *E. dispar* produce **blunt, finger-like pseudopodia** for movement - The pseudopodial morphology is **identical in both species** and cannot be used for differentiation

Question 1

Sabin Feldman dye test is used for diagnosis of which of the following condition?

Accepted Answer

Toxoplasmosis

Answer

Botulism

Answer

Sarcoidosis

Answer

Yellow fever

Question 2

Identify the organism related to the blood smear image.

Accepted Answer

Plasmodium falciparum

Answer

Salmonella Typhi

Answer

Toxoplasma gondii

Answer

Treponema pallidum

Question 3

Which of the following statements about amoebiasis is correct?

Accepted Answer

Amoebic liver abscess occurs in 10% of intestinal amoebiasis cases in tropical regions

Answer

The abscess wall provides the highest yield for Entamoeba histolytica culture

Answer

The portal circulation prevents systemic spread of Entamoeba histolytica

Answer

Amoebic liver abscesses are formed primarily due to bacterial suppuration

Question 4

Which stage of Leishmania is found in spleen aspirate of a patient?

Accepted Answer

Amastigote stage

Answer

Promastigote stage

Answer

Epimastigote stage

Answer

Trypomastigote stage

Question 5

Calabar swelling is produced by?

Accepted Answer

Loa loa

Answer

Brugia malayi

Answer

Onchocerca volvulus

Answer

Wuchereria bancrofti

Question 6

Pulmonary eosinophilia is found in infection with ?

Accepted Answer

Strongyloides

Answer

Babesia

Answer

Malaria

Answer

Trypanosoma

Question 7

Which of the following is the causative organism of Acanthamoeba keratitis?

Accepted Answer

Acanthamoeba

Answer

Plasmodium

Answer

Toxoplasma

Answer

W. bancrofti

Question 8

Promastigote form of Leishmania is found in which part of sandfly?

Accepted Answer

GIT

Answer

Lymph node

Answer

Spleen

Answer

Bone marrow

Question 9

A 52-year-old male with HIV presents with profuse, watery diarrhea of 5 days’ duration. A biopsy of the small intestine is shown here. What is the most likely cause of this patient’s symptoms?

Accepted Answer

Cryptosporidium spp.

Answer

Giardia lamblia

Answer

Cystoisospora belli

Answer

Cyclospora cayetanensis

Question 10

What is a distinguishing feature of E. histolytica compared to E. dispar?

Accepted Answer

Ingestion of red blood cells by trophozoites

Answer

Presence of pseudopodia

Answer

Presence of blunt pseudopodia

Answer

Presence of a thin nuclear membrane

Parasitology — MCQs

Parasitology — MCQs

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