Next-Generation Sequencing in Microbiology Practice Questions

Question 1

Which one of the following is an emerging viral disease?

Accepted Answer

SARS

Answer

Measles

Answer

Chicken pox

Answer

Rabies

Question 2

What is the best investigation for identifying malaria species?

Accepted Answer

Thin smear with Giemsa

Answer

Thick smear

Answer

QBC

Answer

Thin smear with acridine orange

Question 3

Which of the following techniques is primarily used for RNA analysis?

Accepted Answer

Next generation sequencing

Answer

Sanger's technique

Answer

Western blot

Answer

PCR

Question 4

Best method for the detection of mutations with low allele frequency is:

Accepted Answer

Droplet digital PCR

Answer

FISH

Answer

Sanger sequencing

Answer

Nested PCR

Question 5

All of the following are pattern recognition receptors for extracellular or ingested microbes, except:

Accepted Answer

Killer-cell immunoglobulin receptors (KIRs) - Recognize MHC class I molecules on host cells.

Answer

Toll-like receptors (TLRs) - Detect pathogen-associated molecular patterns (PAMPs).

Answer

NOD-like receptors (NLRs) - Intracellular sensors for microbial components.

Answer

C-type lectin receptors (CLRs) - Recognize carbohydrate structures on microbes.

Question 6

Which of the following is a gram-positive organism that shows the following appearance on Ziehl-Neelsen staining?

Accepted Answer

Nocardia

Answer

Mycobacterium tuberculosis

Answer

Actinomyces

Answer

Rhodococcus

Question 7

Gene amplification is achieved through

Accepted Answer

Polymerase Chain Reaction

Answer

DNA strand hybridization

Answer

In situ DNA hybridization

Answer

Ligase chain reaction (LCR)

Question 8

DNA amplification is done by all, except:

Accepted Answer

DNA sequencing

Answer

Loop-mediated isothermal amplification (LAMP)

Answer

Ligase chain reaction

Answer

Polymerase chain reaction

Question 9

Influenza virus causes new epidemic by (3-5 yrs)-

Accepted Answer

Antigenic drift

Answer

Cycle trends

Answer

Mosaicism

Answer

Antigenic shift

Question 10

A microbiologist is working with PCR and wishes to quantify the PCR by monitoring the amplification process while the PCR is ongoing. Which of the following types of PCR should be used?

Accepted Answer

Real-Time PCR (qPCR)

Answer

Standard PCR

Answer

Reverse Transcriptase PCR

Answer

Multiplex PCR

Next-Generation Sequencing in Microbiology — MCQs

Next-Generation Sequencing in Microbiology — MCQs

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