Single Nucleotide Polymorphisms Practice Questions

Question 1

Gene not involved in SCID:

Accepted Answer

BTK

Answer

ZAP70

Answer

IL2RG

Answer

JAK3

Question 2

Which of the following statements accurately describes a gene library?

Accepted Answer

A collection of cloned DNA fragments that represent an organism's genome.

Answer

A physical library containing books about genetics.

Answer

A digital database of genetic sequences.

Answer

A collection of DNA sequences or fragments.

Question 3

Which of the following is used to detect abnormal gene sequences EXCEPT?

Accepted Answer

Flow cytometry

Answer

RFLP analysis

Answer

Pyrosequencing

Answer

FISH

Question 4

Which type of mutation can act as a suppressor to restore the wild-type phenotype in organisms carrying a mutant gene?

Accepted Answer

Mutation of tRNA

Answer

Frameshift mutation of coding gene

Answer

Deletion of mutant gene

Answer

Addition of another normal gene

Question 5

Which of the following statements about polymorphism is true?

Accepted Answer

Single locus with multiple normal alleles.

Answer

Single phenotype linked to a single locus with multiple abnormal alleles.

Answer

Single locus with multiple abnormal alleles, not linked to a specific phenotype.

Answer

Single phenotype linked to a single locus with both normal and abnormal alleles.

Question 6

Which of the following techniques can be used to detect single base pair substitutions?

Accepted Answer

PCR

Answer

FISH

Answer

Southern blot

Answer

Restriction Fragment Length Polymorphism (RFLP)

Question 7

What is the term for a single mutation in a nucleotide base pair that results in a termination codon?

Accepted Answer

Nonsense mutation

Answer

Missense mutation

Answer

Termination mutation

Answer

Silent mutation

Question 8

Which of the following is a primarily RNA based technique?

Accepted Answer

Next generation sequencing

Answer

Sanger's technique

Answer

Western blotting

Answer

PCR

Question 9

All are added to PCR, except:

Accepted Answer

Dideoxynucleotide

Answer

Thermostable DNA polymerase

Answer

Template DNA

Answer

Deoxynucleotide

Question 10

A research team is developing a gene therapy approach using CRISPR-Cas9 to correct a point mutation causing sickle cell disease. They must decide between two strategies: (A) correcting the mutation in hematopoietic stem cells ex vivo, or (B) in vivo correction in bone marrow. Considering molecular physiology principles, what is the most significant advantage of strategy A over strategy B?

Accepted Answer

Strategy A allows for screening and selection of successfully edited cells before transplantation, minimizing off-target effects

Answer

Strategy A requires lower doses of viral vectors

Answer

Strategy A produces faster clinical improvement

Answer

Strategy A is less expensive to implement

Single Nucleotide Polymorphisms — MCQs

Single Nucleotide Polymorphisms — MCQs

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