Molecular Genetics — Flashcards

Flashcard 51: What is the effect of high G-C content on DNA melting temperature?

Answer: high G-C content means high melting temp

Flashcard 52: nucleoside

Answer: base + ribose mnemonic: nucleoSide ends in riboSe

Flashcard 53: nucleotide

Answer: base + ribose + phosphate mnemonic: nucleoTide ends in phosphaTe

Flashcard 54: Identify the nitrogenous base (6-aminopurine):

Answer: Adenine

Extra: Adenine is a purine (6-aminopurine). In DNA, it pairs with Thymine (2 hydrogen bonds). In RNA, it pairs with Uracil.

Flashcard 55: What is the function of topoisomerase in DNA replication?

Answer: Create a nick in DNA helix to allow relaxtion of supercoils

Flashcard 56: What is the function of single stranded binding protein (SSBP) in DNA replication?

Answer: prevents unwound template strands from re-annealing during replication

Flashcard 57: What is the function of helicase in DNA replication?

Answer: unwinds DNA templates at replication fork

Flashcard 58: Which DNA strand undergoes continuous replication? Discontinuous replication?

Answer: leading strand - continuous lagging strand - discontinuous

Flashcard 59: "semiconservative" with respect to DNA

Answer: each DNA molecule contains one old template strand and one new daughter strand

Flashcard 60: origin of replication (ORI) with reference to DNA

Answer: consensus at which DNA replication begins