Molecular Genetics — Flashcards

In an experiment, individual bacterial colonies lack a specific enzyme involed in DNA replication. -This specific enzyme is responsible for removing short fragments of RNA that are base paired to the DNA template. -Which enzymes is most likely deficient in this strain of E. coli?

What are the followings associated with? -Cleavage of DNA strands to remove supercoils -Excision of thymine dimers within DNA -Removal of mismatched base pairs during DNA replication -Removal of RNA primer nucleotides -Unwinding of the 2 strands of template DNA (DNA polymerase 1, DNA gyrase, UV-specific endonuclease, helicase)

What are the two types of DNA mutation that occurs in duchenne muscular dystrophy?

In an experiment, a protein mixture obtained from a cell culture is separated by gel electrophoresis and subsequently transferred to a filter membrane. -Labeled: Double-stranded DNA probes are used to detect a specific protein of interest in the sample. -What protein is most likely to be detected by this method? a.) Ras b.) C-Jun c.) B1- adrenoreceptor d.) S-100 e.) Adenylate cyclase

5 y/o boy comes with progressive muscle weakness that has resulted in numerous recent falls. -Physical exam: Bilateral calf enlargement. -He uses his hands and arms to help push himself to an upright position. -Genetic analysis reveals a point mutation within exon 48 of the gene encoding this muscle protein. -This patient's gene mutation has most likely resulted in which mRNA codon changes?

A group of investigators is studying the mechanisms involved in cancer pathogenesis. -They find that interaction of a certain growth factor with its receptor leads to the following sequence of event: 1. Binding of growth factor 2. Autophosphorylation of tyrosine residues 3. Activation of phosphoinositide 3-kinase 4. Activation of protein kinase B (Akt) 5. Activation of X What is the most likely direct effect of X upon activation?

Describe these 2 genetics term? -Transition -Transversion

Both Prader-Willi and Angelman syndrome are due to a mutation or deletion of genes on chromosome _____

Is tuberous sclerosis inherited or sporadic? _____

Which cell cycle phase(s) are NOT part of interphase? _____