Molecular Genetics — Flashcards

Flashcard 311: Thymidylate Synthase is an enzyme that catalyzes the methylation of _____ to _____, utilizing N5,N10-methylene THF as the carbon donor.

Answer: dUMP

Flashcard 312: What type of probe is used to bind protein when using a Western blot? _____

Answer: Labeled antibody

Extra:   Watch Blotting [https://dashboard.sketchy.com/study/medical/courses/medical-biochemistry/units/medical-biochemistry-molecular-biology/videos/medical-biochemistry-molecular-biology-recombinant-dna-and-biotechnology-blotting?utm_source=anki&utm_medium=partnership&utm_campaign=february_update&utm_content=medical]Watch associated Bootcamp video [https://app.bootcamp.com/med-school/genetics/videos/laboratory-techniques?index=4] https://onlinemeded.org?ref=anki 

Flashcard 313: Down syndrome presents with _____ serum PAPP-A

Answer: decreased

Extra:   Watch Down Syndrome [https://dashboard.sketchy.com/study/medical/courses/medical-biochemistry/units/medical-biochemistry-clinical-genetics/videos/medical-biochemistry-clinical-genetics-trisomy-diseases-downs-syndrome?utm_source=anki&utm_medium=partnership&utm_campaign=february_update&utm_content=medical]Watch associated Bootcamp video [https://app.bootcamp.com/med-school/genetics/videos/high-yield-genetic-disorders?index=6] https://onlinemeded.org/spa/genetics?ref=anki Atlas: 

Flashcard 314: _____ is a lysosomal storage disease caused by frameshift mutations

Answer: Tay-Sachs

Extra:  Watch DNA Mutations [https://dashboard.sketchy.com/study/medical/courses/medical-biochemistry/units/medical-biochemistry-molecular-biology/videos/medical-biochemistry-molecular-biology-dna-dna-mutations?utm_source=anki&utm_medium=partnership&utm_campaign=february_update&utm_content=medical]Watch associated Bootcamp video [https://app.bootcamp.com/med-school/biochemistry/videos/lysosomal-storage-diseases?index=3] https://onlinemeded.org/spa/metabolism?ref=anki 

Flashcard 315: DNA/RNA polymerase always build in the _____ direction

Answer: 5' to 3'

Extra:   https://onlinemeded.org?ref=anki 

Flashcard 316: A(n) _____ transgenic mouse has had a gene removed

Answer: knock-out

Extra:   https://onlinemeded.org?ref=anki 

Flashcard 317: Which cell cycle phase is characterized by DNA synthesis and replication? _____

Answer: S

Extra:   Watch Cell Cycle [https://dashboard.sketchy.com/study/medical/courses/medical-biochemistry/units/medical-biochemistry-cell-biology/videos/medical-biochemistry-cell-biology-cell-cycle-and-division-cell-cycle?utm_source=anki&utm_medium=partnership&utm_campaign=february_update&utm_content=medical]Watch associated Bootcamp video [https://app.bootcamp.com/med-school/genetics/videos/mitosis-meiosis-and-the-cell-cycle?index=5] https://onlinemeded.org/spa/genetics?ref=anki 

Flashcard 318: What enzyme catalyzes the conversion of UDP to dUDP? _____

Answer: Ribonucleotide reductase

Extra:    https://onlinemeded.org/spa/metabolism?ref=anki  

Flashcard 319: DNA laddering in multiples of 180 BP is a sensitive indicator of _____

Answer: apoptosis

Extra:   https://onlinemeded.org/spa/inflammation-and-neoplasia/apoptosis/acquire?ref=anki 

Flashcard 320: Hemophilia A can arise from a(n) _____ mutation without any family history (20%)

Answer: new (de novo)

Extra: Approximately 1/3 of cases of Hemophilia A are due to de novo mutations, meaning there is no prior family history. Note: Acquired Hemophilia A is a distinct clinical entity caused by autoantibodies against Factor VIII; it is associated with pregnancy, autoimmune diseases (e.g., SLE, RA), and certain drugs (e.g., PCN).