CRISPR-Cas9 and Genome Editing — Flashcards

Blue-white assay used in the screening of recombinant vectors for inserted DNA fragments, is based on the principle of _____

In Homologous Recombination, dsDNA breaks are recognized by the _____ protein and the FA Core Complex, which activate BRCA1/2 to recruit enzymes to repair the break

Cloning: After cDNA is produced, cDNA fragments are inserted into _____, which contain antibiotic resistance genes

A _____ is bacteria with plasmids containing DNA fragments representing the majority of the genetic information from a plant or animal.

Which forms of DNA repair repairs double-stranded breaks (due to ionizing radiation)?_____ and homologous recombination

H1 is _____ tightly bound to chromatin and can be removed by adding salt solution.

The promoter is an AT-rich upstream sequence with _____ and CAAT boxes

_____ mosaicism occurs when a mutation arises from mitotic errors after fertilization and propagates through multiple tissues or organs

FISH may be carried out in Metaphase or _____phase of the cell cycle.

_____ test is a widely used biological assay to assess the mutagenic potential of chemical compounds.